The Mostoslavsky Lab Publishes New Platform to Make T Cells from iPSCs

A robust method of producing mature T cells from iPSCs is needed to realize their therapeutic potential. NOTCH1 is known to be required for the production of hematopoietic progenitor cells with T cell potential in vivo. Here we identify a critical window during mesodermal differentiation when Notch activation robustly improves access to definitive hematopoietic progenitors with T/NK cell lineage potential. Low-density progenitors on either OP9-hDLL4 feeder cells or hDLL4-coated plates favored T cell maturation into TCRab+CD3+CD8+ cells that express expected T cell markers, upregulate activation markers, and proliferate in response to T cell stimulus. Single-cell RNAseq shows Notch activation yields a 6-fold increase in multi-potent hematopoietic progenitors that follow a developmental trajectory toward T cells with clear similarity to post-natal human thymocytes. We conclude that early mesodermal Notch activation during hematopoietic differentiation is a missing stimulus with broad implications for producing hematopoietic progenitors with definitive characteristics.

Click here to access the full article

Share with friends

Twitter
LinkedIn
Facebook
WhatsApp
Email

Leave a Reply

Your email address will not be published. Required fields are marked *

Related News

Welcome to CReM

My name is Gabrielle. I'm the Administrative Assistant at CReM. Leave us a short message down below. We will get back to you ASAP!

Our research focuses on understanding how lung epithelial progenitors interact with their supportive niche in the contexts of pulmonary disease and repair. We use cell culture, mouse models, and cell engraftment to study and augment these interactions with the ultimate goal of establishing novel therapies for pulmonary disease.

In addition, it is our mission to foster a welcoming and supportive environment, both within our lab and the wider research community, in order to train the next generation of scientists. We strongly believe that a diverse and collaborative community is an essential foundation for exploring the world around us and developing effective treatments for patients.